A non-invasive method to assess environmental contamination with avian pathogens: beak and feather disease virus (BFDV) detection in nest boxes

A blocking ELISA for the detection of antibodies to psittacine beak and feather disease virus (BFDV).

Currently, the only diagnostic test available routinely for the sero-diagnosis of BFDV is the haemagglutination-inhibition (HI) assay. This test, whilst useful and applicable to samples from a wide range of psittacine birds, is not an ideal assay; it requires erythrocytes from live animals, virus purified from the feathers of infected birds and polyclonal antibody preparations in order to perfo...

Molecular detection and characterization of beak and feather disease virus in psittacine birds in Tehran, Iran

Beak and feather disease virus (BFDV), a member of genus circovirus, is a small, non-enveloped, single stranded DNA virus. Although BFDVs are among the most well studied circoviruses, there is little to no information about BFDVs in Iran. The aim of the present study was to detect and identify BFDV molecules from the birds referred to the avian clinic of The Faculty of Veterinary Medicine, Tehr...

Development of Novel Diagnostic and Vaccine Options for Beak and Feather Disease Virus (BFDV)

PCR-based assays for the detection of BFDV DNA are in widespread use throughout the world. Quan-titative real-time PCR assays are extremely sensitive and have the advantages over standard PCRassays that they do not require post-reaction processing to visualise PCR products and can quan-tify the amount of DNA present in a sample. This study describes a quantitative real-time PCR assa...

Detection of Beak and Feather Disease Virus and Avian Polyomavirus Dna in Psittacine Birds in Poland

The first survey on the incidence of beak and feather disease virus (BFDV) and avian polyomavirus (APV) among the captive psittacine birds in Poland was presented in this study. Samples were collected between 2006 and 2009. A total number of 751 symptom–free birds from 31 genera and 59 species were tested for BFDV and APV by means of a PCR assay. BFDV was found in 25.3% and APV in 22.24% of the...

Psittacine beak and feather disease in Iran, molecular and histopathologic detection